Combining metabolic flux analysis tools and 13C NMR to estimate intracellular fluxes of cultured astrocytes
✍ Scribed by Ana P. Teixeira; Sónia Sá Santos; Nuno Carinhas; Rui Oliveira; Paula M. Alves
- Book ID
- 116765091
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 322 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0197-0186
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
2D [ 13 C, 1 H] COSY NMR is used by the metabolic engineering community for determining 13 C 13 C connectivities in intracellular compounds that contain information regarding the steady-state fluxes in cellular metabolism. This paper proposes innovations in the generation and analysis of these speci
## Abstract At present two alternative methods are available for analyzing the fluxes in a metabolic network: (1) combining measurements of net conversion rates with a set of metabolite balances including the cofactor balances, or (2) leaving out the cofactor balances and fitting the resulting free