Combining DNA and protein vaccines for early life immunization against respiratory syncytial virus in mice
✍ Scribed by Xavier Martinez; Xiaomao Li; Jiri Kovarik; Michel Klein; Paul-Henri Lambert; Claire-Anne Siegrist
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 192 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0014-2980
No coin nor oath required. For personal study only.
✦ Synopsis
Early life responses to respiratory syncytial virus (RSV)-F DNA and RSV-F protein immunization were studied in murine models of neonatal immunization. RSV-F DNA induced similar antibody (Ab) responses, antigen-specific IFN-+ production and cytotoxic T lymphocyte (CTL) responses in 1-week-old and adult BALB/c mice. In contrast , RSV-F protein induced much higher IL-5 responses in early life. Both vaccines elicited Ab and CTL responses in spite of maternal Ab, but with distinctive kinetics. Sequential RSV-F DNA priming/protein boosting primed 1-week-old mice for RSV-F-specific CTL responses, reduced IL-5 production and enhanced Ab responses. In contrast, IL-5 exceeded IFN-+ responses when young mice were primed with protein and boosted with DNA. Last, when protein and DNA immunization were combined, a single vaccine dose induced early Ab responses, preferential IL-5 responses but strong CTL responses. Sequential or combined DNA/protein immunization thus represent interesting strategies for early life immunization.