## Abstract We report on capillary isoelectric focusing‐mass spectrometry (CIEF‐MS) of complex peptide mixtures in the absence of carrier ampholytes. Furthermore, the use of low concentrations of carrier ampholytes as mere spacers is investigated. Carrier ampholytes are complex mixtures of amphoter
Combining capillary electrophoresis with mass spectrometry for applications in proteomics
✍ Scribed by David C. Simpson; Richard D. Smith
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 337 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract We introduce capillary electrophoresis‐mass spectrometry (CE‐MS) as an efficient means for the on‐line separation and identification of protein mixtures. It was found that while CE/electrospray ionization (ESI)‐MS analysis of whole‐cell lysate was too complicated for the one‐dimensional
## Abstract We propose an approach designed to reconstitute a metabolic pathway composed of multistep biochemical reactions, rather than to dissect the individual reactions that make up the pathway. A synthetic __in vitro__ glycolysis was reconstructed from ten purified __Escherichia coli__ (__E. c