## Abstract The detection of disseminated tumor cells in peripheral blood from colorectal cancer patients by RTโPCR could be an attractive method for selecting patients for adjuvant therapy. We here report on realโtime RTโPCR assays (LightCycler) to quantitate potential mRNA markers. We investigate
Combined use of positive and negative immunomagnetic isolation followed by real-time RT-PCR for detection of the circulating tumor cells in patients with colorectal cancers
โ Scribed by Junming Guo; Bingxiu Xiao; Xinjun Zhang; Zhijin Jin; Jian Chen; Lijun Qin; Xiongying Mao; Guangyu Shen; Hui Chen; Zhong Liu
- Publisher
- Springer
- Year
- 2004
- Tongue
- English
- Weight
- 293 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0946-2716
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## Abstract The purpose of our study was to develop specific, sensitive, objective assays for early detection of disseminated tumour cells in patients with colorectal cancer (CRC). Carcinoembryonic antigen (CEA) and cytokeratin 20 (CK20) were chosen as markers because they are selectively expressed