Combined Use of Differential Pulse Polarography and Multivariate Curve Resolution: As Applied to the Study of Metal Mixed Complexes of the Metallothionein Related Hexapeptide
✍ Scribed by M. Silvia Díaz-Cruz; Jesús Mendieta; Miquel Esteban
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 124 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
The complexation of the hexapeptide Lys-Cys-Thr-Cys-Cys-Ala (FT), peptidic fragment {56 ± 61} of mouse liver metallothionein MT I, in the presence of both Cd(II) and Zn(II) is investigated as a model to understand the metalbinding properties of natural MT×s, in which Cd and Zn are simultaneously present. The influence of Cd(II) or Zn(II) on the complexing behavior of FT with Zn(II) or Cd(II), respectively, is studied using the combined techniques of differential pulse polarography and multivariate curve resolution with alternating least square optimization (MCR-ALS). MCR-ALS allows both the unitary voltammograms for each electrochemical process and the concentration profiles of the different species present in the system to be obtained during the experiment, thereby providing a rough picture of the processes involved. Our results allow us to propose the formation of Cd 2 ZnFT 2 and CdZn 2 FT 2 complexes.