✦ LIBER ✦
Combined size-exclusion—ion-exchange high-performance liquid chromatographic analysis of parietaria pollen extracts with the aid of isoelectrofocusing and radio-allergo-sorbent test
✍ Scribed by Ezio Bolzacchini; Giuseppina Di Gregorio; Micaela Nali; Bruno Rindone; Stefano Tollari; Paolo Falagiani; Graziella Riva; Giuseppe Crespi
- Book ID
- 104145833
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 488 KB
- Volume
- 397
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
✦ Synopsis
Ion-exchange high-performance liquid chromatography (HPLC) of the total extract of the pollen of Parietaria judaica allows the separation of several allergens in the acidic pl range and could be used for enrichment procedures. Prior treatment by size-exclusion HPLC improves the efficiency of the purification slightly, allowing further fractionation of a peak at about 12 500 daltons. Isoelectrofocusing and radio-allergo-sorbent test are used for the analysis of individual fractions.