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Combined Protein A and size exclusion high performance liquid chromatography for the single-step measurement of mAb, aggregates and host cell proteins

✍ Scribed by Gjoka, Xhorxhi; Schofield, Mark; Cvetkovic, Aleksandar; Gantier, Rene

Book ID
126888700
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
868 KB
Volume
972
Category
Article
ISSN
1873-376X

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✍ N. Torabi-Pour; A. M. E. Nouri; D. Perrett; R. T. D. Oliver πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 197 KB πŸ‘ 2 views

HPLC was used in combination with immuno-bead separation technique for identification of an individual protein from a pool of proteins. This was carried out using an in-house monoclonal antibody (ATC2) specific for placental alkaline phosphatase (PLAP) as a primary antibody for conjugation to CNBr b