Combined point mutations in codon 12 and 13 of KRAS oncogene in prostate carcinomas
β Scribed by Fatma Silan; Yener Gultekin; Sinem Atik; Davran Kilinc; Cabir Alan; Fazilet Yildiz; Ahmet Uludag; Ozturk Ozdemir
- Book ID
- 106471192
- Publisher
- Springer
- Year
- 2011
- Tongue
- English
- Weight
- 209 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0301-4851
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Detection of point mutations in the Dunning System with the Ki-ras2 gene in the first and second positions of codon 12 exon I, has been performed using allele-specific oligonucleotide (ASO) primers. PCR generated 94bp templates and genomic DNA extracted from the Dunning cell lines AT-1, AT-3, Mat-Lu
## Abstract There is significant evidence that the __ras__ oncogene plays a role in experimental mammary carcinogenesis; the evidence in human breast cancer, however, is more limited. We induced the expression of transformation phenotypes in the human breast epithelial cell line MCFβ10F with the ch