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Combined chromosome microdissection and comparative genomic hybridization detect multiple sites of amplified DNA in a human lung carcinoma cell line

✍ Scribed by Takahiro Taguchi; George Z. Cheng; Daphne W. Bell; Binaifer Balsara; Zemin Liu; Jill M. Siegfried; Joseph R. Testa

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 137 KB
Volume: 20
Category: Article
ISSN: 1045-2257
DOI: 10.1002/(sici)1098-2264(199710)20:2<208::aid-gcc13>3.0.co;2-m

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✦ Synopsis

Chromosome microdissection-fluorescence in situ hybridization and comparative genomic hybridization (CGH) were performed in parallel to identify the native location of amplified DNA in a human non-small cell lung cancer (NSCLC) cell line exhibiting a homogeneously staining region (hsr) and double minutes (dmin). The native locations of microdissected DNA from the hsr and dmin were 7p12-13 and 8q24, respectively. Southern analysis revealed coamplification of EGFR (7p12) and MYC (8q24). CGH detected amplification of DNA not only from 7p12-13 and 8q24, but also from 9p24 and 10q22.

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