𝔖 Bobbio Scriptorium
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Combination of three techniques for the study of complex enzyme mixtures: polyacrylamide gel electrophoresis, immunodiffusion and detection of enzymatic activity

✍ Scribed by Martti Nummi; Arja Lappalainen


Book ID
118387554
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
745 KB
Volume
11
Category
Article
ISSN
0165-022X

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A Method for Activity Staining after Nat
✍ Jean Rivoal; Christopher R. Smith; Trevor F. Moraes; David H. Turpin; William C. πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 129 KB

We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m