Combination of synthetic peptides derived from bone morphogenetic proteins and biomaterials for medical applications

Application of reversible biotinylated l

Application of reversible biotinylated label for directed immobilization of synthetic peptides and proteins: isolation of ligates from crude cell lysates

✍ H. L. Ball; P. Mascagni 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 182 KB 👁 1 views

Chaperonin 10 protein from Rattus norvegicus (Rat cpn10) has been reported to bind chaperonin 60 from Escherichia coli (GroEL) in an ATP-dependent manner. Chemically synthesized Rat cpn10 was immobilized in a defined orientation to agarose-bound monomeric avidin using a reversible biotinylated affin