Combination of single molecule FRET spectroscopy with optical tweezers: A powerful tool for mechanistic studies of enzymes

[Methods in Enzymology] Single Molecule

[Methods in Enzymology] Single Molecule Tools, Part B:Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods Volume 475 || Combining Optical Tweezers, Single-Molecule Fluorescence Microscopy, and Microfluidics for Studies of DNA–Protein Interactions

✍ Gross, Peter 📂 Article 📅 2010 🏛 Elsevier 🌐 English ⚖ 822 KB

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme as