Column chromatography of the bromo—mercuri—methoxy adducts of fatty acid methyl esters as a means of isolating polyenoic acids present in low concentrations
✍ Scribed by J.D. Craske; R.A. Edwards
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 670 KB
- Volume
- 53
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
Due to the extreme complexity of oils such as animal tallows, determination of fatty acid composition by direct gas chromatographic examination of their unfractionated methyl esters is of limited utility. A more detailed analysis of animal fats has been achieved using a silicic acid column chromatographic method to resolve into classes of differing degree of unsaturation, the bromo-mercuri-methoxy adducts of the methyl esters. Methyl esters were recovered from the separated adduct fractions by treatment with acid and analysed by gas chromatography.
This technique should be of general use in those cases where it is necessary to isolate small amounts of dienoic and polyenoic fatty acids from fats, as the required components are protected from autoxidative degradation during the isolation procedure, 'cc OF RR;oMO-i\IeRCUlzI-METH'OSY ADDUCTS OF FATTY ACID METHYL ESTERS 6. ~. . 'l'Al3LEl.V