Column chromatographic separations of amino acids, peptides, and protein in normal human urine
โ Scribed by Paul B. Hamilton; Marjorie F. Lou
- Book ID
- 115766113
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 651 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0006-2944
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๐ SIMILAR VOLUMES
## Abstract A method is presented for analysis of protein amino acids in five minutes, including derivatization. The chemical treatment involves reaction with ethyl chloroformate: if the composition of the reaction medium is optimum this reagent esterifies carboxylic groups simultaneously with deri
The trinitrobenzenesulfonic acid (TNBS) method of R. Fields (1971, Biochem. J., 124, 581-590) has been modified for the manual detection of amino acids and peptides in chromatographic column effluent by changing the reaction conditions to 1 mM TNBS in 0.4 M potassium borate buffer, pH 9.2, at room