✦ LIBER ✦
Colorimetric determination of dinitrophenylamine acids with cyanide ion in dipolar aprotic solvents
✍ Scribed by J.A. Vinson; L.D. Pepper
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 321 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Dinitrophenyl (DNP)-amino acids have been calorimetrically determined by making use of the red color formed by their reaction with cyanide in dimethy~suifoxide or dimethyiacetamide.
The extinction coefficients are over 20,000, making this method more sensitive than previous methods which have used the intrinsic yellow color of DNP-amino acids. Experiments with model compounds have demonstrated that the red cotor is due to the nucleophili~ attack of cyanide on the activated aromatic ring to form a stable anion.