Colorimetric determination of cephradine, a cephalosporin antibiotic

✍ Scribed by Joel Kirschbaum

• Book ID: 102402729
• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 283 KB
• Volume: 63
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600630624

✦ Synopsis

The quantitative colorimetric determination of microgram quantities of cephradine is detailed. An aqueous solution of this compound is reacted with sodium hydroxide, partially neutralized, and subsequently reacted with 5,5'-dithiobis(2-nitrobenzoic acid); the absorbance of the resulting yellow solution is then measured. Other cephalosporins tested give a similar color. Studies with various cephalosporins showed that the development of maximum color intensity was attributable to the presence of the R-CHNHz-CO-cephalosporin nucleus, in which R is a mono-, di-, or trienyl cyclohexyl ring. Molar absorptivities of penicillin solutions tested by the same procedure were found to be 5-15% of those of the cephalosporins.

Keyphrases [7 Cephradine-colorimetric analysis Cephalosporins and penicillins-colorimetric determination Penicillins and cephalwporins-colorimei 'ic determination 5,5'-Dithiobis(2nitrobenzoic acid)-reagent for colorimetric determination of cephalosporins and penicillins Colorimetry-analysis, cephalosporins and penicillins 1 Aldrich Chemical Co.

* A Beckman DU spectrophotometer was used.