𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Colorimetric determination of catecholamines by 2,3,5-triphenyltetrazolium chloride

✍ Scribed by Nawal A. El-Rabbat; Nabil M. Omar

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
368 KB
Volume
67
Category
Article
ISSN
0022-3549

No coin nor oath required. For personal study only.

✦ Synopsis

A convenient spectrophotometric method was developed for the determination of epinephrine, levarterenol, isoproterenol, and methyldopa by reduction of 2,3,5-triphenyltetrazolium chloride and subsequent measurement of the formazan at 485 nm. With absolute alcohol as the solvent, maximum color absorption was attained in 30 min at 25 degrees in the presence of 0.1 N KOH. Evidence is provided to account for the reduction of the tetrazolium salt at the expense of the epinephrine catechol moiety. In addition to the considerably high values of the molar absorptivities of the chromogen formed, ideal adherence of the color absorption to the Beer-Lambert law permitted a sensitive microdetermination of these catecholamines in both pure forms and pharmaceutical formulations. The tetrazolium interaction was selective. No interference was encountered from common catecholamine antioxidants, adjuvants, or noncatechol degradation products.

πŸ“œ SIMILAR VOLUMES

A reversible component of cerebral injur
A reversible component of cerebral injury as identified by the histochemical stain 2,3,5-triphenyltetrazolium chloride (TTC)
✍ D. J. Cole; J. C. Drummond; E. A. Ghazal; H. M. Shapiro πŸ“‚ Article πŸ“… 1990 πŸ› Springer-Verlag 🌐 English βš– 800 KB

The extent of histochemical change following middle cerebral artery occlusion was quantitatively determined in three groups of Sprague-Dawley rats with 2,3,5-triphenyltetrazolium chloride (a marker of mitochondrial oxidative enzyme function). In group I (n = 7) occlusion was maintained for 3 h, with

Reduction of 2,3,5-triphenyltetrazolium
Reduction of 2,3,5-triphenyltetrazolium chloride by the KCN-insensitive, salicylhydroxamic acid-sensitive alternative respiratory pathway of mitochondria from cultured grapevine cells
✍ J. M. Zapata; C. Salinas; A. A. CalderΓ³n; R. MuΓ±oz; A. Ros BarcelΓ³ πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 728 KB

The reduction of 2,3,5-triphenyltetrazolium chloride (TTC) by grapevine cells cultured in suspension was studied in order to assess the reliability of using TTC reduction as a measure of cell viability. Similar to the reduction observed in animals cells, TTC can be reduced in grapevine cells by the

The succinic dehydrogenase activity of t
The succinic dehydrogenase activity of the grasshopper egg and embryo and the colorimetric triphenyltetrazolium chloride method
✍ Bodine, Joseph Hall ;Fitzgerald, Lawrence R. πŸ“‚ Article πŸ“… 1949 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 129 KB

COM &l U N I C A TI 0 N S C'ominents relating to articles which have recently appeared in the Journal of Cellular and Comparative Physiology and lirief descriptions of important observations will be published promptly in this Section. Preliminary announcements of material which will he presented lat

PVC Matrix Membrane Sensor for Potentiom
PVC Matrix Membrane Sensor for Potentiometric Determination of Triphenyltetrazolium Chloride and Ascorbic Acid
✍ Gamal Abdel-Hafiz Mostafa πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 189 KB πŸ‘ 1 views

## Abstract The construction and performance characteristic of a potentiometric PVC membrane sensor for the determination of triphenyltetrazolium chloride (TPTCl) is described. The sensor is based on the use of triphenyltetrazolium (TPT)‐tetraphenylborate (TPB) ion pair as ion exchange sites in PVC