Colorimetric Detection of DNA by Modulation of Thrombin Activity on Gold Nanoparticles
β Scribed by Jyun-Wei Jian; Prof. Chih-Ching Huang
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 628 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0947-6539
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Gold nanoparticles (AuNPs) exhibit distinct optical properties under specific conditions. For example, spherical AuNPs of 13 nm in diameter appear red when well dispersed and blue or purple when aggregated. [1, 2] Two major methods used to control DNA-modified AuNP aggregation are DNA-templated asse
## Abstract We report a highly sensitive method for detecting DNA hybridization by anodicβstripping voltammetry, using assemblies of AuNPs as electrochemical labels. The assemblies are made by layerβbyβlayer modification of subβmicrometer latex spheres, followed by the uptake of the negatively char