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Colorimetric assay to determine alcohol dehydrogenase activity

✍ Scribed by Joan Fibla; Roser Gonzàlez-Duarte

Book ID
116098175
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
371 KB
Volume
26
Category
Article
ISSN
0165-022X

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Assay of glutaminase activity by colorim
Assay of glutaminase activity by colorimetric determination of glutamate
✍ F. Javier Gella; M. Angeles Pascual 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 305 KB

An assay method for glutaminase is described in which the rate of release of glutamate from glutamine is followed in a second step by means of two coupled reactions involving glutamate dehydrogenase and the phenazine methosulfate-catalyzed reduction of iodonitrotetrazolium chloride by NADH. The assa