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Colorimetric assay for lignin peroxidase activity determination using methylene blue as substrate

✍ Scribed by Denise Bello Magalhães; Maria Eleonora Andrade Carvalho; Elba Bon; Júlio Silva Araújo Neto; Sérgio Hélio Kling


Book ID
104654525
Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
277 KB
Volume
10
Category
Article
ISSN
0951-208X

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A kinetic thermometric method was developed for the determination of peroxidase by its catalytic effect on the oxidation of catechol by hydrogen peroxide. The proposed method allows the determination of peroxidase activity over the range 0.05-0.3 I.U. with an R.S.D. of 2.3%. The method was applied t