Colloidal gas aphrons generated from the anionic surfactant AOT for the separation of proteins from aqueous solution
✍ Scribed by Matthew J Noble; Julie Varley
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1999
- Tongue
- English
- Weight
- 174 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0268-2575
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✦ Synopsis
Colloidal gas aphrons are microbubbles 10-100 lm in diameter created by intense stirring of a surfactant solution. They are composed of a gaseous inner core surrounded by a surfactant ülm. They have a high surface area, are relatively stable and have the ability to interact with proteins through electrostatic interactions. These characteristics make colloidal gas aphrons ideally suited for use as a downstream processing technique. In this study the eþ ect of diþ erent process parameters on the recovery of three proteins (conalbumin, ribonuclease A and a-chymotrypsinogen A) was studied for a protein-AOT (sodium bis-(2 ethylhexyl) sulfosuccinate) system. High enrichment ratios (up to 8) and high recovery of the protein (85% ) are found under optimum conditions.