Colloid titration of heparin using Cat-Floc (polydiallyldimethyl ammonium chloride) as standard polycation
✍ Scribed by Takeshi Katayama; Ken-ichi Takai; Reiko Kariyama; Yasuhiro Kanemasa
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 266 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
The positive polymer, Cat-Floe, was used for colloid titration. Colloid titration with Cat-Floe was not affected by pH between 2 and 12. The polymer combined with heparin, forming a flocculant, and the binding was shown to be stoichiometric by gravimetric analysis of heparin-sulfur.
Results showed that heparin could be measured quantitatively by colloid titration with Cat-Floe. The applicability of this method for measurements of other biochemical materials was discussed.
Colloid titration is a method for quantitative analysis of a negative or positive colloid solution which is titrated by an oppositely charged colloid solution. Originally (I), chitosan or N-methylchitosan (macramine) was used as a standard positive colloid solution and potassium polyvinylsulfate (PVSK) was used as a standard negative colloid solution (Fig. 1). Senju (2) improved the method by using N-methylglycolchitosan (MGch) instead of N-methylchitosan as the standard polycation to obtain more constant blank titration values over a wide pH range, although the titration values are changed slightly at different pH values. Recently TGei and Kawada (3) proposed polydiallyldimethylammonium chloride (Cat-Floe) as the standard polycation. The reagent is highly polymerized and it contains only quartet-nary ammonium groups, but no primary, secondary, or tertiary amines.
This paper describes the determination of heparin solution by colloid titration using Cat-Floe and PVSK as the standard polycation and polyanion, respectively. It was found that heparin bound to Cat-Floe stoichiometrically.
Methods
Chemicals.