✦ LIBER ✦

Collection and X-ray microanalysis of airway surface liquid in the mouse using ion exchange beads

✍ Scribed by Harriet Nilsson; Inna Kozlova; Viengphet Vanthanouvong; Godfried M Roomans

Publisher: Elsevier Science
Year: 2004
Tongue: English
Weight: 195 KB
Volume: 35
Category: Article
ISSN: 0968-4328
DOI: 10.1016/j.micron.2004.03.005

No coin nor oath required. For personal study only.

✦ Synopsis

The airway surface liquid (ASL) is a thin layer of liquid covering the airway epithelium. The ionic composition of the ASL is assumed to be important for airway function and may be altered in diseases such as cystic fibrosis and exercise-induced asthma. A method for collection of ASL is presented in which the fluid is collected using small dextran ion-exchange beads. The beads are equilibrated with the ASL in a humidity chamber, collected under silicon oil, dried and analyzed by X-ray microanalysis. Analysis of standard beads prepared by exposure to different salt solutions shows that linear calibration lines can be obtained, but that beads absorb different elements to a different extent. The results show that the ASL in mice is hypotonic, and that the mucus component of the ASL has an elemental composition that is different from that of the periciliary fluid.

📜 SIMILAR VOLUMES

Composition of nasal airway surface liqu

Composition of nasal airway surface liquid in cystic fibrosis and other airway diseases determined by X-ray microanalysis

✍ V. Vanthanouvong; I. Kozlova; M. Johannesson; E. Nääs; S.L. Nordvall; A. Dragomi 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 253 KB 👁 1 views

The ionic composition of the airway surface liquid (ASL) in healthy individuals and in patients with cystic fibrosis (CF) has been debated. Ion transport properties of the upper airway epithelium are similar to those of the lower airways and it is easier to collect nasal ASL from the nose. ASL was c

Use of freeze-substitution and molecular

Use of freeze-substitution and molecular distillation drying in the preparation of Dunaliella parva for ion localization studies by X-ray microanalysis

✍ Dr. M. A. Hajibagheri; T. J. Flowers 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 593 KB

The determination of ion concentrations within cells and sub-cellular compartments remains a difficult procedure, as the volumes to be analyzed are rather small. X-ray microanalysis is sufficiently sensitive, and has adequate resolution, to measure these concentrations. The major difficulties are re

Implications of specimen preparation and

Implications of specimen preparation and of surface contamination for the measurement of the grain boundary carbon concentration of steels using x-ray microanalysis in an UHV FESTEM

✍ Cousens, D. R.; Wood, B. J.; Wang, J. Q.; Atrens, A. 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 161 KB 👁 1 views

The purpose of the present investigation was to gain an understanding of the nature of the carbon contamination on the surface of standard steel transmission electron spectroscopy (TEM) specimens, the effect of exposure of a clean specimen to normal laboratory air, and the efficacy of plasma-cleanin