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Collection and culture of alveolar bone marrow multipotent mesenchymal stromal cells from older individuals

✍ Scribed by Juan Han; Hironori Okada; Hideki Takai; Youhei Nakayama; Takahide Maeda; Yorimasa Ogata

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
237 KB
Volume
107
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

In this work, we examined the culture condition of alveolar bone marrow multipotent mesenchymal stromal cells (ABMMSCs), aiming to apply regenerative therapy to older periodontitis patients. To better understand the character of cultured cells from alveolar bone marrow, the expression profiles of well‐known genes and their responses to the induction of osteogenic, chondrogenic, or adipogenic differentiation were examined. Using Ξ±MEM‐based culture, ABMMSCs could be obtained from older individuals than in previous reports. Interestingly, ABMMSCs expressing Klf4 were able to differentiate into osteoblasts. The prediction of differentiation potential by Klf4 could be a useful guide for further improvement of the culture conditions required to culture ABMMSCs derived from older individuals. J. Cell. Biochem. 107: 1198–1204, 2009. Β© 2009 Wiley‐Liss, Inc.

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