✦ LIBER ✦

Collagenase induced changes in the circular dichroism spectrum of collagen

✍ Scribed by F. H. Chu; A. Lukton

Publisher: Wiley (John Wiley & Sons)
Year: 1974
Tongue: English
Weight: 356 KB
Volume: 13
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1974.360130713

No coin nor oath required. For personal study only.

✦ Synopsis

The maximum at 220 nm in the circular dichroism spectrum of native collagen solution changed to a negative value after heat denaturation or collagenase hydrolysis. The enzyme induced rate of C1) change a t 220 nm was shown to be first order in collagen concentration. The specific rate constant k is actually a combined rate constant kfast and k,l,,v in which the ratio k,/k, is 4.1. The initial rates were linear with respect t o enzyme concentration, and the I<, was found to be 3.5 X 10-7 Ill. The rate of ultraviolet hyperchromicity at 220 nm on collagen hydrolysis was determined. The kf3't was the same as that obtained by C1).

Both methods may be readily wed to assay for collagenase activity.

The k,/k, ratio was 4.6.

📜 SIMILAR VOLUMES

Circular dichroism of collagen, gelatin,

Circular dichroism of collagen, gelatin, and poly(proline) II in the vacuum ultraviolet

✍ Duane D. Jenness; Cindy Sprecher; W. Curtis Johnson Jr. 📂 Article 📅 1976 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 463 KB 👁 2 views

## Abstract Circular dichroism spectra for acid‐soluble calfskin collagen, gelatin, and poly(proline) II in solution have been extended into the vacuum ultraviolet region. The extended spectrum of gelatin reveals that the circular dichroism of this unordered polymer is more closely related to the s

Chromophore Alignment in a Chiral Host P

Chromophore Alignment in a Chiral Host Provides a Sensitive Test for the Orientation – Intensity Rule of Induced Circular Dichroism

✍ Xiangyang Zhang; Werner M. Nau 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 159 KB 👁 1 views

Circular dichroism is an essential spectral property for probing chirality. 2] An interesting effect arises when an achiral guest chromophore is complexed in a chiral host. The guest becomes optically active, a phenomenon referred to as ªinducedº circular dichroism (ICD). [3±5] The spectroscopic in

Correlation of fluorescence and circular

Correlation of fluorescence and circular dichroism spectroscopy with electrospray ionization mass spectrometry in the determination of tertiary conformational changes in calcium-binding proteins

✍ Timothy D. Veenstra; Kenneth L. Johnson; Andy J. Tomlinson; Rajiv Kumar; Stephen 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 173 KB 👁 2 views

We compared changes in the fluorescence, circular dichroism (CD) and multiply charged electrospray ionization mass spectrometry (ESI-MS) spectra of three calcium (Ca 2 )-binding proteins upon the binding of Ca . The proteins used were rat brain calbindin D 28K and two deletion mutants, one lacking E