Collagen-like polypeptides: Synthesis of radioactive polytripeptides with the internal sequence -Gly-Pro-Pro-

✍ Scribed by Elijah Adams; N. Venkateswara Rao; Samboornam Ramaswamy

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: French
• Weight: 688 KB
• Volume: 15
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580150149

✦ Synopsis

The synthesis of the tripeptide polymers (Pro-Gly-Pro) n, (Pro-Pro-Gly) n, and (Gly-Pro-Pro),, is described, with particular attention to procedures which favor the efficient insertion of radioactive proline in either of the two proline positions 'X" or "Yr in the internal sequence, Gly-X-Y. By our methods, radioactive proline in the X-position is most efficiently obtained as (Pro-Gly-*Pro)n, while radioactive proline in the Y-position is most efficiently obtained as (Gly-Pro-ProIn. Some of the properties of these polymers and the stability of ' H -and "C polymers are discussed. An additional finding relates to the synthesis of proline-containing tripeptides via the N-hydroxysuccinimide esters: our experience suggests the generalization that a peptide bond is not readily formed between pyrrolidine amino acids when a dipeptide is involved in such coupling reactions.