✦ LIBER ✦

Collagen immobilised on silica derivatives as a new stationary phase for HPLC

✍ Scribed by Maciej Turowski; Roman Kaliszan

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 135 KB
Volume: 12
Category: Article
ISSN: 0269-3879
DOI: 10.1002/(sici)1099-0801(199807/08)12:4<187::aid-bmc727>3.0.co;2-2

No coin nor oath required. For personal study only.

✦ Synopsis

Synthesis of new stationary phases containing covalently bound collagen has been described. Commercially available soluble collagen and the silica derivatives, aminopropylsilica (APS) and diol-silica, were used for the experiment. The products of synthesis were subjected to elemental analysis and to a 13C-NMR analysis to prove the presence of the covalently bound protein on the support's surface. The stationary phases were packed into the columns and introduced into an HPLC system. A series of diversified test compounds was analysed to elucidate the retention mechanism operating on the collagen column by means of the quantitative structure-retention relationship (QSRR) analysis. Chromatographic analysis of a series of selected compounds was performed for which human skin permeation data were available. Quantitative structure-activity relationship (QSAR) analysis was done to model the human skin permeation by employing of the chromatographic data determined on the collagen column. The newly obtained collagen phases were demonstrated to possess distinctive retention properties due to a combination of specific (polar) and hydrophobic solute-stationary phase interactions. The normal-phase retention mechanism seemed to prevail on the collagen phases. For the set of test solutes available the interactions with collagen appear to be of secondary importance for their ability to permeate human skin compared to their hydrophobicity and binding to keratin. None the less, the collagen columns may be of value to complete the chromatographic model of human skin permeation.

📜 SIMILAR VOLUMES

Separation of benzodiazepines on a new c

Separation of benzodiazepines on a new carbohydrate-based chiral stationary phase for HPLC

✍ Apryll M. Stalcup; Wenhong Wu; Karen L. Williams 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 167 KB 👁 2 views

Several benzodiazepines were enantioresolved on a new carbohydrate chiral stationary phase based on maltooligosaccharides. The role of organic modifier, ionic strength, pH and temperature are examined and the results are discussed. In general, selectivity and retention were found to decrease with in

Chiral recognition mechanism for the res

Chiral recognition mechanism for the resolution of enantiomers on a highly effective HPLC chiral stationary phase derived from (R)-4-hydroxyphenylglycine

✍ Myung Ho Hyun; Chung-Sik Min 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 191 KB 👁 1 views

A chiral stationary phase (CSP 1) prepared starting from (R)-4hydroxyphenylglycine and then grafting (R)-N-butanoyl-4-allyloxyphenylglycine Npropyl amide to silica gel was found to be very effective in separating the enantiomers of N-(3,5-dinitrobenzoyl)-␣-amino amides. From the chromatographic beha

New Stationary Phase Prepared by Immobil

New Stationary Phase Prepared by Immobilization of a Copper-Amine Complex on Silica and Its Use for High Performance Liquid Chromatography

✍ Silva, Cesar R. ;Jardim, Isabel C. S. F. ;Airoldi, Claudio 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 91 KB 👁 2 views

Chromatographic silica (10 lm) was chemically modified with the silylating agent: [3-(2-aminoethyl)aminopropyl]trimethoxysilane (AEAPTS). The reaction product was characterized by elemental analysis and infrared and 13 C and 29 Si NMR spectra. The chemically modified silica was treated with Cu(II) i

HPLC enantioseparation on cellulose tris

HPLC enantioseparation on cellulose tris(3,5-dimethylphenylcarbamate) as a chiral stationary phase: Influences of pore size of silica gel, coating amount, coating solvent, and column temperature on chiral discrimination

✍ Eiji Yashima; Pennapa Sahavattanapong; Yoshio Okamoto 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 444 KB 👁 1 views

Cellulose tris(3,5dimethylphenylcarbamate) (CDMPC) was coated on largepore silica gels and used as a chiral stationary phase (CSP) for high-performance liquid chromatographic separation of enantiomers. The influences of pore size of silica gel, coating amount of CDMPC, coating solvent, and column te

(S)-Leucine and [(S)-1-(1-Naphthyl)ethyl

(S)-Leucine and [(S)-1-(1-Naphthyl)ethyl]amine as Chiral Building Blocks for a Bifunctional System − Synthesis of a New Chiral Stationary Phase and Evaluation of Its Biselector Properties in the HPLC Resolution of Racemic Compounds

✍ Anna Iuliano; Emanuele Attolino; Piero Salvadori 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 295 KB 👁 2 views

Two selectors derived from [(S)-1-(1-naphthyl)ethyl]amine and (S)-leucine have been chemically bonded to one another and the resulting chiral auxiliary linked covalently to silica gel, to produce a new chiral stationary phase (CSP) for the HPLC resolution of enantiomers. The CSP is able to separate

Chiral separation of some 4a-methyl-1,2,

Chiral separation of some 4a-methyl-1,2,3,4,4a,9a-hexahydro-fluoren-9-one derivatives as a probe for difference in solvation by 2-propanol of carbamate moiety in chiralcel OD-H, chiralpak AD, and chiralpak AS chiral stationary phases

✍ Christian Roussel; Cristina Suteu; Latifa Shaimi; Mohammed Soufiaoui; Brice Bonn 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 372 KB

Chromatographic resolution of 12 derivatives in the 4a-methyl-2,3,4,4atetrahydro-1H-fluorene and 4a-methyl-1,2,3,4,4a,9a-hexahydrofluoren-9-one series differing by the framework around position 9 and substitution in position 6, are reported on Chiralcel OD, Chiralpak AD, and Chiralpak AS under two e