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Collagen at interfaces I.In situ collagen adsorption at solution/air and solution/polymer interfaces

✍ Scribed by Deyme, Michel ;Baszkin, Adam ;Proust, Jacques Emile ;Perez, Eric ;Boissonnade, Marie Martine

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
678 KB
Volume
20
Category
Article
ISSN
0021-9304

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✦ Synopsis

Collagen was isolated from rat tail tendons and acetylated with '-I4C acetic anhydride.

In situ adsorption of this collagen from a buffer solution (pH = 2.7) was measured at the interfaces to air, polyethylene and polyethylene grafted with poly(ma1eic acid), respectively.

The kinetics of adsorption were recorded for all surfaces studied and the corresponding diffusion coefficients for collagen in solution with various protein concentrations were calculated. The desorption of collagen from polymer surfaces was also studied.

These experiments reveal the existence of both a reversibly and an irreversibly adsorbed collagen layer on the polymers tested. The desorptionladsorption ratio for the polyethylene is higher than that for the grafted polyethylene indicating stronger interactions of collagen with the grafted surface than with the non-modified polyethylene.

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