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Colchicine-resistance and enhancement of P-glycoprotein activity after co-cultivation of drug-sensitive cells with multidrug resistant variants.

✍ Scribed by A.V. Eliseenkova; E.S. Kakpakova; R.I. Abdrjashitov; A.A. Stavrovskaya

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
286 KB
Volume
19
Category
Article
ISSN
1065-6995

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✦ Synopsis

The role of cellular interactions in the resistance of Djungurian hamster cells to colchicine (CH) and in the efficiency of P‐glycoprotein function was studied. Mixtures of CH‐resistant and CH‐sensitive cells as well as control unmixed cells were propagated for 3 days and the sensitivity of the cells to CH was measured by colony forming assay. Identification of individual subpopulations was possible due to genetic marker (6TG‐resistance). The data show that the survival of CH‐sensitive cells in CH‐supplemented medium increased after co‐cultivation with CH‐resistant counterparts. To measure Pgp activity the fluorescent dye RH123 and FACScan analysis were used. Pgp‐mediated RH123 efflux increased after co‐cultivation of CH‐sensitive and CH‐resistant cells.

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