Colchicine-induced heritable variation in cell size and chloroplast number in the leaf cells of inbred ryegrasses (Lolium perenne, L. multiflorum)
✍ Scribed by Lutful Hassan; R. N. Jones; J. S. Parker; U. K. Posselt
- Book ID
- 104617461
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 436 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0014-2336
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✦ Synopsis
A 3 hr period of treatment with a 0 .2% aqueous solution of colchicine was given to one week old seedlings of 3 inbred lines of perennial ryegrass (Lolium perenne) and 4 lines of Italian ryegrass (L . multiflorum) . The surviving plants developed as mixoploids and were self-pollinated . In the following generation (CT1) the seeds were grown without further treatment, and were screened cytologically at the seedling stage to separate out diploids from tetraploids . Only the diploids were kept, and in this CT1 generation comparisons were then made between controls (2x) and colchicine-treated diploids (C2x) for leaf mesophyll cell size and chloroplast number variation . Heritable differences were found such that the cell plan areas and/or the numbers of chloroplasts per cell were significantly greater in the C2x compared with the 2x treatment in 3 and 5 out of the 7 lines . In each case there was enhancement of chloroplast numbers independently of the variation in cell size .