(Coixan polysaccharide)-graft-Polyethylenimine Folate for Tumor-Targeted Gene Delivery

✍ Scribed by Qiying Jiang; Peihua Shi; Chunyan Li; Qingqing Wang; Fujian Xu; Wantai Yang; Guping Tang

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 550 KB
• Volume: 11
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.201000350

✦ Synopsis

Abstract

CP‐PEI‐FA was prepared as an effective vector for in vitro and in vivo tumor‐targeted gene delivery. The structures of the polymers were characterized, and their DNA condensation capability, particle sizes, zeta potentials, cytotoxicity and in vitro/in vivo transfection were examined. The cytotoxicity of CP‐PEI‐FA was significantly lower than that of PEI 25 kDa and close to that of PEI 1200. The in vitro transfection of CP‐PEI‐FA was tested in C6 and HeLa cells (FR‐positive cells) and A549 cells (FR‐negative cells). CP‐PEI‐FA showed a high targeting specificity and good gene transfection efficiency in FR‐positive cells. These results indicate that CP‐PEI‐FA is a safe and effective polyplex‐forming agent for both in vitro and in vivo transfection of plasmid DNA. magnified image