Coherent anti-Stokes Raman scattering microscopy based on spectral encoding

✍ Scribed by Eun Seong Lee; Jae Yong Lee

• Book ID: 104074076
• Publisher: Elsevier Science
• Year: 2009
• Tongue: English
• Weight: 331 KB
• Volume: 282
• Category: Article
• ISSN: 0030-4018
• DOI: 10.1016/j.optcom.2009.01.041

✦ Synopsis

We present a theoretical description of spectrally encoded coherent anti-Stokes Raman scattering (SE-CARS) microscopy. Conceptually, two broadband laser beams, whose center frequencies differ by the Raman shift of the sample to be measured, are diffracted with equal dispersions by a single grating and form a focal line on the sample plane. The CARS signal is generated in such a way that its spectral profile reflects the spatial distribution of sample molecules with the same vibrational frequencies. The spectral point spread functions for a Raman shift of 2850 cm À1 are calculated to estimate the number of resolvable points.