Cofactor-containing bioluminescent fibre-optic sensor: new developments with poly(vinyl alcohol) matrices
✍ Scribed by Sabrine M. Gautier; Loïc J. Blum; Pierre R. Coulet
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 488 KB
- Volume
- 255
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
A cofactor-containing bioluminescent fibre-optic sensor for mcotmamide adenine dmucleotide (NADH) is described The oxidoreductase-luciferase membrane placed at the tip of the fibre-optic ensures the specific molecular recognition of NADH and, in the presence of both a flavor cofactor [flavor mononucleotide (FMN)] and an aldehydic substrate, light emission occurs The combination of immobilized enzymes and entrapped co-reactants would allow the biosensor to operate in a reagentless way In a first step to design a self-contained biosensor, the flavor cofactor was entrapped in poly(vmyl alcohol) matrices and was internally released in the vicinity of the bound enzymes, whereas the aldehyde was continuously supplied in the reaction medium The non-covalent unmobilization of the flavor in translucent matrices was performed by entrapment in either a photo-cross-linked poly(vmyl alcohol) (PVA) network or a PVA matrix reticulated with glutaraldehyde The performance of the biosensor was evaluated with regard to sensitivity (detection limit), dynamic linear range and self-containment