Codelivery to Mammalian Cells of a Transcriptional Factor with cis-Acting Element Using Cationic Liposomes

✍ Scribed by H. Farhood; X. Gao; J. Barsoum; L. Huang

• Book ID: 102561073
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 439 KB
• Volume: 225
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1995.1112

✦ Synopsis

The human immunodeficiency virus-1 transactivator protein (tat) was codelivered efficiently with a reporter gene under the control of a tat-responsive DNA element using different formulations of cationic liposomes. Expression of a tat-responsive reporter gene was induced by incubating cells with a mixture of purified recombinant tat protein, reporter DNA, and liposomes. Different cell lines were tested successfully as targets for the codelivery. Tat was shown to trans-activate the codelivered virus promoter specifically in the cells tested. Codelivery of tat with DNA is a useful model for studying the function of trans-acting factors and their cisacting DNA elements. The currently available methods such as foot-printing only reveal the binding, but not the functional consequence of the binding, of the factor with the element. In addition, this system may prove useful as a model for high level and regullated transgene expression in target cells. 1995 Academic Press, Inc.