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CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design

✍ Scribed by Rose, Timothy M. (author);Henikoff, Jorja G. (author);Henikoff, Steven (author)

Book ID
124055237
Publisher
Oxford University Press
Year
2003
Tongue
English
Weight
234 KB
Volume
31
Category
Article
ISSN
0305-1048

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Searching for nitrile hydratase using th
Searching for nitrile hydratase using the Consensus-Degenerate Hybrid Oligonucleotide Primers strategy
✍ Pedro Miguel Lopes Lourenço; Tânia Almeida; Diogo Mendonça; Fernanda Simões; Car 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 185 KB

## Abstract Consensus‐Degenerate Oligonucleotide Hybrid Primers (CODEHOP) were designed by using the WWW‐implemented strategy, based on multiple alignments of nitrile hydratase (NHase) __α__ subunit, available from EMBL database. These primers were successfully tested with known NHase‐producing bac