Cobalt phthalocyanine as a mediator for the electrooxidation of glucose oxidase at glucose electrodes

✍ Scribed by I. Rosen-Margalit; A. Bettelheim; J. Rishpon

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 754 KB
• Volume: 281
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)85189-q

✦ Synopsis

A method for the construction of a highly stable enzyme electrode is described. A new mediator, cobalt phthalocyanine, a biocompatible material, was immobilized together with the enzyme glucose oxidase in a colloidal graphite emulsion matrix. The resulting glucose electrode was characterized. The electrode retains its full activity for long periods (more than 11 months). The response to glucose is high, fast and independent of pH (5-8) or oxygen partial pressure. The linear range obtained (O-5 mM) can be extended by the introduction of a dialysis membrane and can thus be adjusted to the measurement of glucose concentrations in the range of medical interest. The electrode was tested successfully on serum samples.