Coaxial Continuous-flow Fast-atom Bombardment vs Electrospray Ionization: a Sensitivity Comparison on a Magnetic Sector Instrument
✍ Scribed by Leesa J. Deterding; Morteza Khaledi; Kenneth B. Tomer
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 656 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0951-4198
No coin nor oath required. For personal study only.
✦ Synopsis
The performance of an electmspray (ESI) source was evaluated and compared to that of a coaxial continuousflow fast-atom bombardment source on a magnetic mass spectrometer using ten dflerent peptides over the mass range of 400 to 3500 Da and using a variety of scanning modes. Results show that sensitivities using the two ionization techniques are similar, with limits-of-detection in the attomole to low femtomole range. In addition, proteins can be routinely detected using ESI at femtomole levels. The observance of the noncovalent complex of RNase A and cytidine 2'-monophosphate yields evidence that these complexes can be studied using instruments that operate at high accelerating voltages.