## Abstract This study attempted to investigate whether different levels of mitotic activity exist within different physical regions of a human embryonic stem (hES) cell colony. Incorporation of 5‐bromo‐2‐deoxyuridine (BrdU) within newly‐synthesized DNA, followed by immunocytochemical staining was
✦ LIBER ✦
Co-culture of human embryonic stem cells with murine embryonic fibroblasts on microwell-patterned substrates
✍ Scribed by Ali Khademhosseini; Lino Ferreira; James Blumling III; Judy Yeh; Jeffrey M. Karp; Junji Fukuda; Robert Langer
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 615 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0142-9612
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## Abstract Realizing the potential clinical and industrial applications of human embryonic stem cells (hESCs) is limited by the need for costly, labile, or undefined growth substrates. Here we demonstrate that trypsin passaging of the hESC lines, HUES7 and NOTT1, on oxygen plasma etched tissue cul