CMR2009: 11.01: In vivo detection of DIACEST contrast agent labeled liposomes using MRI

✍ Scribed by G. Liu; Y. E. Har-el; C. Long; A. Gilad; J. Zhang; G. Sgouros; J. W. M. Bulte; P. C. M. van Zijl; M. T. McMahon

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 71 KB
• Volume: 4
• Category: Article
• ISSN: 1555-4309
• DOI: 10.1002/cmmi.354

✦ Synopsis

Results: Characterization of FeO-HDL revealed close resemblance to native HDL, in terms of physical properties and cholesterol efflux capacity. In vitro experiments showed that the particles were avidly taken up by macrophages (Fig. ). In vivo experiments on apoE-KO mice revealed a substantial signal decrease in the liver and lesioned vessel wall on T 2 Ã -weighted MR images (Fig. ). Ex vivo results confirmed the presence of FeO-HDL in atherosclerotic plaques using optical imaging (Fig. and), while TEM on liver tissue revealed trafficking of the HDL within the hepatocyte and macrophage cells and localization of the HDL particles in cellular compartments. Conclusions: The HDL mimicking iron oxide nanoparticle platform is a highly flexible contrast agent that closely mimics the natural composition and properties of endogenous HDL. This nanoparticulate contrast agent enabled us to perform an extensive and multimodality investigation of receptor mediated interactions and metabolism of high density lipoproteins in atherosclerotic disease.