Cloud-point extraction and capillary electrochromatography: An approach for the analysis of selected environmental toxicants in spiked human serum
✍ Scribed by Sarath R. Sirimanne; John R. Barr; Donald G. Patterson Jr.
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 189 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1040-7685
No coin nor oath required. For personal study only.
✦ Synopsis
The use of cloud-point extraction CPE coupled with capillary elec-Ž . trochromatography CEC as an approach for the analysis of environmental toxicants in human serum was explored. Human serum samples spiked with polycyclic aromatic hydrocarbons, polychlorinated dibenzo-p-dioxins, or phthalates were extracted using Genapol X-080 and 25% NaCl at 46ЊC for 5 min. The surfactant-rich phase was treated with acetonitrile to remove some of the interfering coextractants and then analyzed by CEC. The analytes within these three groups were well separated via this technique even though the presence of the residual coextractantsrsurfactant shifted the migration time slightly. The use of CPE-CEC proved to be a viable approach for environmental toxicant analysis. In addition, both CPE and CEC require relatively small amounts of organic solvents compared with conventional sample preparation techniques, and thus are more environmentally friendly.