Osmotin purified from the latex of Calot
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Cleverson Diniz Teixeira de Freitas; Fábio César Sousa Nogueira; Ilka Maria Vasc
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Article
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2011
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Elsevier Science
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English
⚖ 505 KB
A protein, similar to osmotin- and thaumatin-like proteins, was purified from Calotropis procera (Ait.) R.Br latex. The isolation procedure required two cation exchange chromatography steps on 50mM Na-acetate buffer (pH 5.0) CM-Sepharose Fast Flow and 25 mM Na-phosphate buffer (pH 6.0) Resource-S, r