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Closure strategies for random DNA sequencing

โœ Scribed by EDWARDS, A; CASKEY, C

Book ID
123114964
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
716 KB
Volume
3
Category
Article
ISSN
1046-2023

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โœ Yu-May Lee; Sheng-Chung Lee ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 492 KB

A modification of Lin's systematic DNA sequencing strategy is described. A method based on the religation of compatible cohesive ends generated by Sau3AI and BamHI was developed. The original procedure has been simplified and the yield of transfectant has been greatly improved. After complete digest