Closing the 1,3-propanediol route enhances hydrogen production from glycerol by Halanaerobium saccharolyticum subsp. saccharolyticum

✍ Scribed by Anniina Kivistö; Ville Santala; Matti Karp

• Publisher: Elsevier Science
• Year: 2011
• Tongue: English
• Weight: 572 KB
• Volume: 36
• Category: Article
• ISSN: 0360-3199
• DOI: 10.1016/j.ijhydene.2011.03.012

✦ Synopsis

The effect of vitamin B 12 on hydrogen (H 2 ) and 1,3-propanediol (1,3-PD) production in glycerol fermentation of Halanaerobium saccharolyticum subsp. saccharolyticum was studied in batch cultivations. In addition, the effect of vitamin B 12 on glucose fermentation of H. saccharolyticum subsp. saccharolyticum and on glycerol fermentation of H. saccharolyticum subsp. senegalensis, that is a non-1,3-PD producing bacterium, and of Clostridium butyricum, that is known for vitamin B 12 -independent 1,3-PD production, were studied for comparison. 1,3-PD production of H. saccharolyticum subsp. saccharolyticum was observed to be vitamin B 12 -dependent and the H 2 production in glycerol fermentation was remarkably enhanced when 1,3-PD production was blocked by the lack of vitamin B 12 supplementation.

The highest H 2 yield obtained by H. saccharolyticum subsp. saccharolyticum was 2.16 mol/mol glycerol. No significant effects of vitamin B 12 on metabolite production in glucose fermentation of H. saccharolyticum subsp. saccharolyticum or in glycerol fermentation of H. saccharolyticum subsp. senegalensis or C. butyricum were observed.