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Cloning the Bacillus subtilis 168 aroC gene encoding dehydroquinase

โœ Scribed by Warburg, R.J.; Mahler, I.; Tipper, D.J.; Halvorson, H.O.


Book ID
120220855
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
915 KB
Volume
32
Category
Article
ISSN
0378-1119

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We have devised a method whereby any mutagenized cloned DNA from Bacillus subtilis can be reinserted at the original site on the B. subtilis chromosome. The procedure depends on the accuracy and high frequency of homologous recombination between the B. subtilis chromosome and the DNA taken up by the