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Cloning, Soluble Expression and Purification of High Yield Recombinant hGMCSF in Escherichia coli

✍ Scribed by Das, Krishna M.P.; Banerjee, Sampali; Shekhar, Nivedita; Damodaran, Karpagavalli; Nair, Rahul; Somani, Sandeep; Raiker, Veena P.; Jain, Shweta; Padmanabhan, Sriram

Book ID
120192485
Publisher
Molecular Diversity Preservation International
Year
2011
Tongue
English
Weight
374 KB
Volume
12
Category
Article
ISSN
1422-0067

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Recombinant human interleukin-6 (hIL-6), a pleiotropic cytokine containing two intramolecular disulfide bonds, was expressed in Escherichia coli as an insoluble inclusion body, before being refolded and purified in high yield providing sufficient qualities for clinical use. Quantitative reconstituti