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Cloning, sequencing and expression in Escherichia coli of a thermophilic lipase from Bacillus thermoleovorans ID-1

✍ Scribed by A-Ra Cho; Seung-Ku Yoo; Eui-Joong Kim

Book ID
117516355
Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
330 KB
Volume
186
Category
Article
ISSN
0378-1097

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Cloning and expression of a thermophilic
Cloning and expression of a thermophilic Ξ±-amylase gene from Bacillus stearothermophilus in Escherichia coli
✍ Tsukagoshi, N. ;Ihara, H. ;Yamagata, H. ;Udaka, S. πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 584 KB

A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII