Cloning, sequencing and amplification of the alkaline extracellular protease (XPR2) gene of the yeast Yarrowia lipolytica
โ Scribed by Nicaud, J.-M.; Fabre, E.; Beckerich, J.M.; Fournier, P.; Gaillardin, C.
- Book ID
- 122058611
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 700 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0168-1656
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The mating type gene MATA of the dimorphic yeast Yarrowia lipolytica was cloned. The strategy used was based on the presumed function of this gene in the induction of sporulation. A diploid strain homozygous for the mating type B was transformed with an integrative gene bank from an A wild-type stra
The secretion and maturation of the acid extracellular protease (AXP) of the yeast Yarrowia lipolytica have been characterized using antiserum raised against this enzyme. A 42 kDa pro-enzyme form of AXP was identified from lysates of radiolabelled Y. lipolytica cells and found to contain no N-linked