Cloning, overexpression, purification, a
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Alexey Gribenko; Maria M. Lopez; John M. Richardson III; George I. Makhatadze
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Article
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1998
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Cold Spring Harbor Laboratory Press
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English
β 459 KB
The calcium-binding protein S100P has been found to be associated with human prostate cancer. We have overexpressed S100P in Escherichia coli using a T7 expression system. A rapid two-step procedure for the isolation of overexpressed S100P leads to a preparation of >95% pure protein with a yield of