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Cloning, overexpression and purification of functionally active Saccharomyces cerevisiae Hop1 protein from Escherichia coli

โœ Scribed by Krishnendu Khan; T.P. Vipin Madhavan; K. Muniyappa


Book ID
116829140
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
640 KB
Volume
72
Category
Article
ISSN
1046-5928

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โœ Itaya, Mitsuhiro ;McKelvin, Dorothy ;Chatterjie, Sunil K. ;Crouch, Robert J. ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 881 KB

We have cloned genes encoding RNase H from Escherichia coli rnh mutants, Salmonella typhimurium and Saccharomyces cerevisiae. Selection was accomplished by suppression of the temperature-sensitive growth phenotype of Escherichia coli strains containing the rnh-339::cat and either recB270 (Ts) or rec