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Cloning of two hexokinase isoenzyme sequences from Drosophila melanogaster

✍ Scribed by P.Cyril Jayakumar; Yogesh S Shouche; Milind S Patole

Book ID
117673016
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
492 KB
Volume
31
Category
Article
ISSN
0965-1748

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Hexokinase isoenzyme PI was cloned using a gene pool obtained from a yeast strain having only one functional hexokinase, isoenzyme PI. The gene was characterized using 20 restriction enzymes and located within a region of 2.0 kbp. The PI plasmid strongly hybridized with the PII plasmids isolated pre